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murine wildtype lifr cdna  (OriGene)


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    OriGene murine wildtype lifr cdna
    OP9 and NIH/3T3 cells show differential levels of OSMR and <t>LIFR.</t> ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.
    Murine Wildtype Lifr Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+wildtype+lifr+cdna/Lifr+(NM_013584)+Mouse+Tagged+ORF+Clone/pmc08584221-202-0-8
    Average 90 stars, based on 1 article reviews
    murine wildtype lifr cdna - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR"

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222111649

    OP9 and NIH/3T3 cells show differential levels of OSMR and LIFR. ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.
    Figure Legend Snippet: OP9 and NIH/3T3 cells show differential levels of OSMR and LIFR. ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.

    Techniques Used: Expressing, Negative Control, Molecular Weight, Labeling, Microarray, Software, Incubation

    OSMR downregulation attenuates OSM effects on proliferation. ( A , B ) Immunoblot quantifying the shRNA-induced knockdown of Osmr (n = 4) and Lifr (n = 5) in ( A ) OP9 and ( B ) NIH/3T3 cells. The shRNAs Osmr 5 and Lifr 1 were used in subsequent experiments. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( C ) Relative quantification of S phase of OP9 and NIH/3T3 cells carrying a knockdown for either Osmr , Lifr, or a Renilla control in presence or absence of 10 ng/mL mOSM. Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. *** p < 0.001. ns = not significant. ( D ) Immunoblot quantifying the LIFR overexpression in OP9 and NIH/3T3 cells. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( E ) Lifr overexpressing OP9 and NIH/3T3 cells were examined for LIFR surface expression compared to cells carrying the mock control. ( F ) Relative quantification of S phase of OP9 and NIH/3T3 cells transduced with Lifr or empty vector (MiG). Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: OSMR downregulation attenuates OSM effects on proliferation. ( A , B ) Immunoblot quantifying the shRNA-induced knockdown of Osmr (n = 4) and Lifr (n = 5) in ( A ) OP9 and ( B ) NIH/3T3 cells. The shRNAs Osmr 5 and Lifr 1 were used in subsequent experiments. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( C ) Relative quantification of S phase of OP9 and NIH/3T3 cells carrying a knockdown for either Osmr , Lifr, or a Renilla control in presence or absence of 10 ng/mL mOSM. Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. *** p < 0.001. ns = not significant. ( D ) Immunoblot quantifying the LIFR overexpression in OP9 and NIH/3T3 cells. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( E ) Lifr overexpressing OP9 and NIH/3T3 cells were examined for LIFR surface expression compared to cells carrying the mock control. ( F ) Relative quantification of S phase of OP9 and NIH/3T3 cells transduced with Lifr or empty vector (MiG). Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Western Blot, shRNA, Knockdown, Molecular Weight, Labeling, Quantitative Proteomics, Control, Cell Cycle Assay, Over Expression, Expressing, Transduction, Plasmid Preparation

    Related Articles

    Expressing:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Negative Control:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Molecular Weight:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Labeling:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Microarray:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Software:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Incubation:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Western Blot:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    shRNA:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Knockdown:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Quantitative Proteomics:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Control:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Cell Cycle Assay:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Over Expression:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Transduction:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].

    Plasmid Preparation:

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR
    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).. The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].The retroviral vector was constructed by cloning the Lifr cDNA into the MigRI retroviral vector coexpressing the enhanced green fluorescent protein (EGFP; a kind gift from W. Pear, Philadelphia, PA, USA) [ ].



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    murine wildtype lifr cdna  (OriGene)
    90
    OriGene murine wildtype lifr cdna
    OP9 and NIH/3T3 cells show differential levels of OSMR and <t>LIFR.</t> ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.
    Murine Wildtype Lifr Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+wildtype+lifr+cdna/Lifr+(NM_013584)+Mouse+Tagged+ORF+Clone/pmc08584221-202-0-8
    Average 90 stars, based on 1 article reviews
    murine wildtype lifr cdna - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    OP9 and NIH/3T3 cells show differential levels of OSMR and LIFR. ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.

    Journal: International Journal of Molecular Sciences

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR

    doi: 10.3390/ijms222111649

    Figure Lengend Snippet: OP9 and NIH/3T3 cells show differential levels of OSMR and LIFR. ( A ) OP9 and NIH/3T3 cells were examined for LIFR and OSMR expression. BaF3 cells were used as a negative control. Protein molecular weight is labeled in kDa. Relative protein quantities are marked in red. ( B ) Microarray analysis of OP9 and NIH/3T3 cells showing Osmr and Lifr expression. The p -values were calculated by Transcriptome Analysis Console software. *** p < 0.001. ns = not significant. ( C ) OP9 and NIH/3T3 cells were analyzed for LIFR surface expression using FACS. ( D ) OP9 and NIH/3T3 cells were examined for expression of LIFR and OSMR in relation to different incubation periods with 10 ng/mL mOSM. Protein molecular weight is labeled in kDa. ( E ) OP9 and NIH/3T3 cells were examined for LIFR surface expression in relation to different incubation periods with 10 ng/mL mOSM. p -Values were calculated using one-way ANOVA and Bonferroni post-test. * p <0.05 and *** p < 0.001. ns = not significant.

    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).

    Techniques: Expressing, Negative Control, Molecular Weight, Labeling, Microarray, Software, Incubation

    OSMR downregulation attenuates OSM effects on proliferation. ( A , B ) Immunoblot quantifying the shRNA-induced knockdown of Osmr (n = 4) and Lifr (n = 5) in ( A ) OP9 and ( B ) NIH/3T3 cells. The shRNAs Osmr 5 and Lifr 1 were used in subsequent experiments. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( C ) Relative quantification of S phase of OP9 and NIH/3T3 cells carrying a knockdown for either Osmr , Lifr, or a Renilla control in presence or absence of 10 ng/mL mOSM. Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. *** p < 0.001. ns = not significant. ( D ) Immunoblot quantifying the LIFR overexpression in OP9 and NIH/3T3 cells. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( E ) Lifr overexpressing OP9 and NIH/3T3 cells were examined for LIFR surface expression compared to cells carrying the mock control. ( F ) Relative quantification of S phase of OP9 and NIH/3T3 cells transduced with Lifr or empty vector (MiG). Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR

    doi: 10.3390/ijms222111649

    Figure Lengend Snippet: OSMR downregulation attenuates OSM effects on proliferation. ( A , B ) Immunoblot quantifying the shRNA-induced knockdown of Osmr (n = 4) and Lifr (n = 5) in ( A ) OP9 and ( B ) NIH/3T3 cells. The shRNAs Osmr 5 and Lifr 1 were used in subsequent experiments. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( C ) Relative quantification of S phase of OP9 and NIH/3T3 cells carrying a knockdown for either Osmr , Lifr, or a Renilla control in presence or absence of 10 ng/mL mOSM. Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. *** p < 0.001. ns = not significant. ( D ) Immunoblot quantifying the LIFR overexpression in OP9 and NIH/3T3 cells. Protein molecular weight is labeled in kDa. Relative protein quantities are indicated in red. ( E ) Lifr overexpressing OP9 and NIH/3T3 cells were examined for LIFR surface expression compared to cells carrying the mock control. ( F ) Relative quantification of S phase of OP9 and NIH/3T3 cells transduced with Lifr or empty vector (MiG). Cell cycle analysis was performed as described before. One-way ANOVA and Bonferroni post-test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Murine wildtype Lifr cDNA (MR221059) was obtained from OriGene (Rockville, MD, USA).

    Techniques: Western Blot, shRNA, Knockdown, Molecular Weight, Labeling, Quantitative Proteomics, Control, Cell Cycle Assay, Over Expression, Expressing, Transduction, Plasmid Preparation